Chosen Solution

The screen got shipped in and I put everything on perfectly and it has a black spot on the bottom, I’m guessing this was from the LOCA or glue used to secure the glass to the lcd as it is a original lcd with a fixed glass but does anyone have any other ideas why this could be happening?.

Actually, that looks like a backlight issue. Nobody uses LOCA anymore and serious refurbishing centers have been using OCA for a long time now. If you bought this screen from iFixit, please contact iFixit support, they will help you out.